Development of new beta-glucosidase activated with its catalytic product

2015 Fiscal Year Final Research Report

• Project/Area Number: 26660081
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: Hokkaido University
• Principal Investigator: MORI HARUHIDE 北海道大学, (連合)農学研究科(研究院), 教授 (80241363)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: β-グルコシダーゼ / バイオマス糖化 / 酵素活性化 / 反応機構

Outline of Final Research Achievements

Enhancement of activity of beta-glucosidase from Streptomyces sp. with the product glucose was investigated.. Kinetics analyses with various substrates and monosaccharides revealed that the activation is caused not only glucose but also other monosaccharides as well as even short-chain substrates with solely drastic increase in kcat. The monosaccharides, partially occupying the far side of substrate binding site from the catalytic site, prevent the short-chain substrates from binding in the non-productive fashion. Based on possible amino acid residues associated to the monosaccharide binding, candidate beta-glucosidases equipped with the activation feature can be selected.

Free Research Field

応用生物化学