Chemical communication between plants and protists: Identification of a factor of Plasmodiophor barssicae to recognize its hosts

2016 Fiscal Year Final Research Report

• Project/Area Number: 26660095
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bioorganic chemistry
• Research Institution: Yamaguchi University
• Principal Investigator: Matsui Kenji 山口大学, 創成科学研究科, 教授 (90199729)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 根こぶ病菌 / 休眠胞子 / 発芽 / ハクサイ / ダイズ / 根滲出液

Outline of Final Research Achievements

In order to elucidate the host specificity of Plasmodiophora brassicae that cause clubroot disease in Brassicae plants, we attempted to identify substances that induced germination of the resting spores of P. brassicae to release zoospores. At first, we purified the resting spores with density gradient centrifugation with Percol, which made us to estimate the germination rate efficiently. Based on the results obtained with the bio-assay, we realized that the substances that induced germination of the resting spores occurred in roots of soybean and oat as well as Chinese cabbage, and that only Chinese cabbage secreted the substances into oil from roots. The substances obtained from Chinese cabbage roots consisted of at least two components with low and high molecular weight. Both of them seemed to be quite unstable in the presence of organic solvent.

Free Research Field

植物代謝生化学