A new non-invasive in vivo model for evaluation of the adipose inflammatory state.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26660112
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Food science
• Research Institution: Hiroshima University
• Principal Investigator: Yanaka Noriyuki 広島大学, 生物圏科学研究科, 准教授 (70346526)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 肥満 / 脂肪組織 / 炎症 / 非侵襲 / 化学発光 / マクロファージ

Outline of Final Research Achievements

The infiltration of macrophages into adipose tissue and their interaction with adipocytes are essential for the chronic low-grade inflammation of obese adipose tissue. In this study, we identified the serum amyloid A3 (Saa3) gene that is affected by interaction with macrophages. We showed that the Saa3 promoter in adipocytes actually responds to activated macrophages in a co-culture system. Decreasing C/EBPβ abundance in 3T3-L1 adipocytes or point mutation of C/EBPβ elements suppressed the increased promoter activity in response to activated macrophages, suggesting an essential role of C/EBPβ in Saa3 promoter activation. Bioluminescence based on Saa3 promoter activity in Saa3-luc mice was promoted in obese adipose tissue, showing that Saa3 promoter activity is most likely related to macrophage infiltration. This study suggests that the level of expression of the Saa3 gene could be utilized for the inflammation of obese adipose tissue.

Free Research Field

分子栄養学