Research for a novel regulatory mechanism of appetite via a des-acyl ghrelin receptor

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670037
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pharmacology in pharmacy
• Research Institution: Kagoshima University
• Principal Investigator: IIJIMA Mikio 鹿児島大学, 医歯学域医学系, 助教 (00305111)
• Co-Investigator(Kenkyū-buntansha): KISHDA Shosei 鹿児島大学, 医歯学域医学系, 教授 (50274064) ; KOYAMA Hirofumi 鹿児島大学, 医歯学域医学系, 講師 (40709656) ; KISHDA Michiko 鹿児島大学, 医歯学域医学系, 助教 (40274089) ; KATO Ikuo 神戸薬科大学, 薬学部, 教授 (70509843)
• Co-Investigator(Renkei-kenkyūsha): INUI Akio 鹿児島大学, 医歯学域医学系, 教授 (80168418)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: デスアシルグレリン

Outline of Final Research Achievements

Ghrelin is a 28-amino acid orexigenic peptide hormone mainly produced by the stomach. The acylation of the third amino residue at ghrelin is required for its binding and activation of the ghrelin receptor. At first des-acyl ghrelin (DAG) was considered an inactive form of ghrelin, but now the DAG is believed to be a biologically active. To clone the DAG receptor(s), we performed a biotinyl DAG binding assay using the mouse adult brain cDNA expression library transfected cells. We succeeded in the identification of a DAG receptor candidate gene. This candidate gene product bound to the biotinyl DAG in vitro. Future study is required to determine the precise mechanism(s) of DAG action.

Free Research Field

細胞生物学