Development of a fluorescence correlation spectroscopy for rotational diffusion measurements

2016 Fiscal Year Final Research Report

• Project/Area Number: 26670086
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Terada Sumio 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (00262022)
• Co-Investigator(Renkei-kenkyūsha): TANII Takashi 早稲田大学, 理工学術院, 教授 (20339708) ; KONDO Takao 名古屋大学, 理学研究科, 教授 (10124223) ; JIN Takashi 理化学研究所, 生命システム研究センター, チームリーダー (80206367)
• Research Collaborator: TANI Tomomi 米国ウッズホール海洋生物学研究所, 研究員 ; KINJO Masataka 北海道大学, 先端生命, 教授
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 顕微鏡技術 / 生体分子間相互作用ネットワーク

Outline of Final Research Achievements

As rotational properties sensitively reflect molecular interactions and intracellular environment, polarization-dependent fluorescence correlation spectroscopy can be a promising method to analyse various cell biological events. We constructed both new polarization-dependent fluorescence correlation spectroscopy and an analysis program for this purpose. In addition, we could develop new methods to label proteins of interests for our analysis.

Free Research Field

解剖学、細胞生物学、生物物理学