2016 Fiscal Year Final Research Report
Polarized fluorescence microscopy analysis of nuclear lamina
| Project/Area Number |
26670087
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
Sato Keisuke 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (60644044)
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| Research Collaborator |
TANI Tomomi 米国ウッズホール海洋学研究所, 研究員
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ラミン / 蛍光偏光 |
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| Outline of Final Research Achievements |
To analyze the structure of Lamin filaments, which is the main constituent of nuclear lamina of eukaryotic cells, human Lamin A and Lamin B1 proteins were constrainedly tagged with EGFP. We tried to replace endogenous Lamin genes of HeLa cells with cDNA of these proteins, and partially succeeded.
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| Free Research Field |
細胞生物学
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