Imaging-based analysis of nodes of Ranvier dynamics

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670120
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General pharmacology
• Research Institution: Institute of Physical and Chemical Research (2015); The University of Tokyo (2014)
• Principal Investigator: Ino Daisuke 国立研究開発法人理化学研究所, 生命システム研究センター, 特別研究員 (40721981)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 有髄神経 / Ranvier絞輪 / イメージング

Outline of Final Research Achievements

Nodes of Ranvier play a pivotal role in fast transmission of sensory- and motor information in the peripheral nervous system, and disruption of nodes of Ranvier can be a cause of various neurological diseases. To gain the insight into how disruption of nodes of Ranvier occurs during the disease progression, we aimed to develop a method that allows analysis of nodes of Ranvier dynamics in the intact tissue. We developed a fluorescent probe that labels nodes of Ranvier. By expressing this probe in mice spinal cord using AAV vector, we analyzed the morphological changes nodes of Ranvier in diseased nerves.

Free Research Field

神経科学