2015 Fiscal Year Final Research Report
Regulatory mechanisms of protein S-polythiolation
| Project/Area Number |
26670131
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Kumamoto University |
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Principal Investigator |
Sawa Tomohiro 熊本大学, 生命科学研究部, 教授 (30284756)
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| Co-Investigator(Kenkyū-buntansha) |
IDA Tomoaki 東北大学, 大学院医学系研究科, 助教 (70570406)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | シグナル伝達 / プロテオミクス / 翻訳後修飾 / 酸化ストレス |
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| Outline of Final Research Achievements |
Protein S-polythiolation is a post-translational modification that introduce additional sulfur atoms on cysteine residues in proteins. We developed specific method that enables biotin-labeling on S-polythiolated cysteines by using methylsulfonyl benzothiazole and cyanide biotin. We successfully identified endogenous S-polythiolated proteins including cytoskeleton proteins. The method we developed may help further understanding of signal transduction mediated via protein S-polythiolation.
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| Free Research Field |
生化学
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