Discovery of Aurora-A Regulated Signal Networks Critical for Glucose Metabolism and EMT

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670159
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pathological medical chemistry
• Research Institution: Okayama University
• Principal Investigator: Katayama Hiroshi 岡山大学, 医歯(薬)学総合研究科, 准教授 (90713975)
• Co-Investigator(Kenkyū-buntansha): Kaori Sasai 岡山大学, 大学院医歯薬学総合研究科, 助教 (50722162)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: オーロラキナーゼ / p53 / EMT / glycolysis

Outline of Final Research Achievements

In this research project, we examined whether Aurora-A oncoprotein involves in EMT and glycolysis which are crucial process for transformation of cells. We revealed that Aurora-A overexpression results in induction of EMT and loss of cell polarity in 3D culture, and enhanced glycolysis during mitosis. Aurora-A phosphorylation of p53 but not p73 also showed similar increase glycolysis. Analysis of proteins specifically interacting to phosphory-mimetic mutant of p53 by Mass-spectrometry revealed the existence of proteins known to involve in the process of glycolysis. Our study suggests that Aurora-A plays an essential role in the tumorigene throuhg dereguation of EMT and glycolyssis which are parlty mediated through p53 phosphorlyation.

Free Research Field

腫瘍生物学