2016 Fiscal Year Final Research Report
Development of histo-in situ technique demonstrating sequencing specific methyalted cytosine
| Project/Area Number |
26670176
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Ehime University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | DNAメチル化 / in situ hybridization / Histochemistry / Epigenetics |
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| Outline of Final Research Achievements |
By making a ICON a probe which specifically cross-link and bound to the methylated cytosine, probe DNA can specifically hybridize to the target DNA in a sequence dependent manner. By PRINS method, a DNA elongation reaction was established using bounded ICON probe on the specific ch romosome. Modified nucleotides were incorporated during this elongation stem and visualized by a conventio nal histochemical techniques. By this easy, methyalation status of specific chromosome or DNA sequence cou Id be visualized. So far we gathered a lot of basic and fundamental data. \Ve are now currently trying to v isualize a single-copy gene during spermatogenesis in mouse testis, and updating our protocol for more sta ble and reliable one.
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| Free Research Field |
分子病理学
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