2015 Fiscal Year Final Research Report
Structure-function studies of bacterial mitoNEET system
| Project/Area Number |
26670215
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KUSANO TERUO 日本医科大学, 医学部, 助教 (30434129)
KUMASAKA TAKASHI 高輝度光科学研究センター, タンパク質結晶解析推進室, 室長代理 (30291066)
IWASAKI HIDEO 早稲田大学, 理工学術院, 教授 (00324393)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | mitoNEET / 鉄硫黄クラスター / 構造生物学 / オミックス解析 / 構造機能 / 鉄硫黄タンパク質 / 好熱菌 |
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| Outline of Final Research Achievements |
MitoNEET is a novel outer-mitochondrial membrane iron-sulfur protein, recently identified as a potential mitochondrial target that binds pioglitazone, an insulin sensitizer for the treatment of type II diabetes. Recently we constructed a null-deletion mutant strain of Thermus thermophilus lacking TthNEET (the thermophile homolog of mitoNEET), and found a “prokaryotic glucose intolerance” for this delta-TthNEET null strain. In this work, we characterized TthNEET mutant proteins and strains with altered [2Fe-2S] cluster redox potentials by X-ray crystallographic and physiological analyses to better understand the structure-function relationships of this class of proteins, and explored the protein interaction network of TthNEET by monitoring the whole-cell global changes of the Thermus protein components, using pulldown assays and two-dimensional gel electrophoresis.
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| Free Research Field |
生化学
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