Study of gliding movement and epithelial-cell adhesion mechanism found in unclassified pnumonic bacteria of CFB phylum

2016 Fiscal Year Final Research Report

• Project/Area Number: 26670217
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including mycology)
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Ike Fumio 国立研究開発法人理化学研究所, バイオリソースセンター, 専任研究員 (40183157)
• Research Collaborator: KAJITA AYAKO 国立研究開発法人理化学研究所, バイオリソースセンター, 人材派遣 ; SAKAMOTO MITSUO 国立研究開発法人理化学研究所, バイオリソースセンター, 専任研究員 (50321766)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: カー(CAR)バチルス / フィロバクテリウム・ローデンティウム / グラム陰性菌 / マウス / 呼吸器感染症 / 肺炎病原菌 / 細胞付着機構 / 滑走運動

Outline of Final Research Achievements

The cilia-associated respiratory bacillus (CARB), formerly unclassified, extracellular, gram-negative filamentous bacterium, colonizes the ciliated respiratory epithelium of rodents and causes persistent respiratory diseases. Previously we improved culture method of CARB of SMR-C strain, extracted genomic DNA from SMR-C and decided its full genome sequences with help of Prof. Hattori and Dr. Oshima at Tokyo University. Genomic analysis showed 40% of annotated genes were not found on the DDBJ/EMBL/Genbank database and we could not speculate virulent factor genes of CARB from its genome.
In this study, we tried to clarify anchoring system of CARB onto epithelial cells, especially focusing on gliding-related genes. Meanwhile we gave scientific name, Filobacterium rodentium, to SMR-C strain also with new genus and family names. We also found F. rodentium displayed stress-induced phase variations including biofilm formation. These findings will help to promote the study of F. rodentium.

Free Research Field

細菌学、実験動物学、感染症