Fabrication of a human iPSC-derived cardiac tissue using 3D bioprinter

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670410
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular medicine
• Research Institution: Keio University
• Principal Investigator: Tohyama Shugo 慶應義塾大学, 医学部, 助教 (90528192)
• Co-Investigator(Kenkyū-buntansha): Fujita Jun 慶應義塾大学, 医学部(信濃町), 特任講師 (10306706) ; Nakamura Makoto 富山大学, 大学院理工学研究部(工学), 教授 (90301803)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: ヒトiPS細胞 / 心筋細胞 / 血管内皮細胞 / 3Dプリンター / スキャフォールド

Outline of Final Research Achievements

Human iPSCs are one of the most attractive cell sources for regenerative medicine. Although many challenges have been overcome for clinical application, establishment of optimal method for transplantation is an unsolved problem. In this study, we aimed to fabricate a human iPSC-derived aligned cardiac tissue with . At first, we have built a grid-like gel scaffold using the 3D bioprinter. Thereafter, we seeded cardiomyocytes and fabricated a mesh-like aligned cardiac tissue. This mesh-like aligned cardiac tissue is expected to be utilized for part of large cardiac tissue or for a tool of drug screening.

Free Research Field

心臓再生医療