Epigenetic Regulation of hematopoietic stem cells by O-GlcNAc transferase

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670469
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: Yokohama City University (2015); Keio University (2014)
• Principal Investigator: NAKAJIMA Hideaki 横浜市立大学, 医学(系)研究科(研究院), 教授 (30217723)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: O結合型β-N-アセチルグルコサミン転移酵素 / 糖鎖修飾 / 造血幹細胞 / 造血前駆細胞 / 胎仔肝

Outline of Final Research Achievements

O-linked-β-N-acetylglucosamine (O-GlcNAc) transferase （OGT） is a critical enzyme that catalyzes O-GlcNAcylation of Ser/Thr residues of various proteins. This study examined the physiological role of OGT in hematopoietic system using OGT conditional knockout mice. OGT-deficient fetal liver cells were severely decreased in number and lost colony-forming activity in methylcellulose culture. Moreover, self-renewal and long-term repopulating capacity of OGT-deficient fetal liver hematopoietic stem cells were severely impaired in transplantation assays. These results indicated that OGT played a critical role in fetal liver hematopoiesis.

Free Research Field

血液学