Analysis of virome in patients with hematopoietic stem cell transplantation

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670495
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pediatrics
• Research Institution: Nagoya University
• Principal Investigator: Kimura Hiroshi 名古屋大学, 医学(系)研究科(研究院), 教授 (30303621)
• Co-Investigator(Kenkyū-buntansha): OKUNO Yusuke 名古屋大学, 医学部附属病院, 特任講師 (00725533) ; ITO Yoshinori 名古屋大学, 大学院医学系研究科, 准教授 (20373491)
• Research Collaborator: KAWADA Jun-ichi 名古屋大学, 大学院医学系研究科, 助教
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: ウイルス / 造血幹細胞移植 / 次世代シーケンサー

Outline of Final Research Achievements

In hematopoietic stem cell transplantation against children, it is essential to control a variety of viruses that sequentially emerge during transplantation. However, we can detect only small part of the iceberg. To clarify the genomes of all the viruses (so-called “virome”) that inhabit patients with stem cell transplantation, we applied next generation sequencing (NGS) to peripheral blood from transplanted recipients. Peripheral blood was sequentially taken from 4 patients with hematopoietic stem cell transplantation, and comprehensive genome sequencing was performed using MiSeq. From patients’ peripheral blood, TT virus, SEN virus, human herpesvirus 6B, and so on were detected by NGS. Interestingly, dynamics of viral load was correlated with clinical courses in some patients.

Free Research Field

ウイルス学