2015 Fiscal Year Final Research Report
Induction and expansion of PSC-derived HSCs by manipulating culture microenvironment
| Project/Area Number |
26670498
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatrics
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NIWA Akira 京都大学, iPS細胞研究所, 助教 (20546999)
Osawa Mitsujiro 京都大学, iPS細胞研究所, 助教 (70546770)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 造血幹細胞 / ES/iPS細胞 / 移植・再生医療 / 臨床応用 / 細胞内代謝 / ストロマ細胞 / ニッチ |
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| Outline of Final Research Achievements |
Developing the way of hematopoietic stem cell (HSC) expansion or de novo stem cell induction has been of great interest in medical scientific fields. We cleared that pluripotent stem cell (PSC)-derived VE-Cadherin+ endothelial cells function as stromal microenvironment to induce PSC-derived HSC-like cells; they showed serial colony forming potential in vivo and were observed engrafted in NOG mice as long as twelve weeks. Moreover, a small molecule cocktail containing GSK3 and AHR pathway inhibitors successfully expanded them as high as six times in vitro, and increased the chimerism in vivo.
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| Free Research Field |
医歯薬学
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