2016 Fiscal Year Final Research Report
Fabrication of biological arterial graft for congenital heart disease
| Project/Area Number |
26670506
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatrics
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| Research Institution | Yokohama City University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MASUDA Munetaka 横浜市立大学, 医学研究科, 教授 (10190365)
MATSUSAKI Michiya 大阪大学, 工学研究科, 助教 (00419467)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 再生医療 / 循環器・高血圧 / 平滑筋 / 人工血管 |
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| Outline of Final Research Achievements |
We previously demonstrated that a layer by layer method using gelatin and fibronectin enabled to fabricate three-dimensional multi-layered smooth muscle cells. Based on this technique, we aimed to fabricate implantable arterial grafts. We optimized density of cell seeding and incubation time and obtained seven-layered multilayered smooth muscle cells that have 0.05 mm in thickness. The burst pressure was 1874±255 mmHg (718-3138 mmHg). We implanted the sheet into rat abdominal aorta as a patch graft and found graft patency for five month.
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| Free Research Field |
循環生理学
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