2016 Fiscal Year Final Research Report
Analysis of a protein localized at stratified epithelial cell junction
| Project/Area Number |
26670527
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NISHIZAWA Yuji 中部大学, 生命健康科学部, 教授 (80252229)
AOYAMA Yumi 川崎医科大学, 医学部, 教授 (90291393)
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| Research Collaborator |
IWATSUKA Tomohiro
MATSUDA Kazuhisa
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 細胞間接着 / 表皮分化 |
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| Outline of Final Research Achievements |
Hemidesmosomes and desmosomes in epidermis contain proteins that are not found in their counterparts in simple epithelia. In this study, we have analyzed a novel cell-cell junction protein that shows unique tissue distribution. Fist, we produced a monoclonal antibody specific to the protein. Using this antibody we demonstrated its differentiation-dependent expression in stratified epithelia. We further analyzed the mechanism that target the protein to cell-cell adhesion site by transient transfections of cDNA constructs encoding various mutants of the protein.
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| Free Research Field |
皮膚科学
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