Identification of cancer initiating cell factors and an attempt of drug development

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670625
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Respiratory surgery
• Research Institution: Nagoya University
• Principal Investigator: SUZUKI Motoshi 名古屋大学, 医学(系)研究科(研究院), 講師 (80236017)
• Co-Investigator(Kenkyū-buntansha): FUKUI Takayuki 名古屋大学, 医学部附属病院, 病院講師 (70463198) ; WAKAHARA Keiko 名古屋大学, 医学部附属病院, 病院講師 (00631433) ; KATO Sei-ichi 名古屋大学, 大学院医学系研究科, 講師 (30584669) ; Nishida Atsushi 千葉大学, 大学院薬学研究院, 教授 (80130029)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 肺がん / がん幹細胞 / 低分子化合物

Outline of Final Research Achievements

We collected clinical lung cancer specimens and transplanted each of in the immune-deficient mice. Xenografts were recovered after tumor formation, collagenase-treated, and sorted for CD166 positive population. In a cell line, the CD166 positive cells successfully formed secondary tumors in mice. We performed database analysis to figure out a gene candidate which may contribute to the cancer initiating cell nature. By using the above cell line, we established a stable cell line that is associated with a knockdown of this gene, and inoculated thousands of cells in mice. Results are forthcoming. We also screened for an inhibitor against this gene product. Among the 2000 candidates, we found a compound that inhibits the activity. To decrease the EC50 value, we started optimization of this compound.

Free Research Field

分子腫瘍学