Direct reprogramming of fibroblasts toward external urethral sphincter for future regenerative therapy in urinary incontinence

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670704
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Oita University
• Principal Investigator: Shin Toshitaka 大分大学, 医学部, 助教 (60404381)
• Co-Investigator(Renkei-kenkyūsha): MIMATA Hiromitsu 大分大学, 医学部, 教授 (60219714) ; SUMINO Yasuhiro 大分大学, 医学部, 客員研究員 (30325716) ; SHINOHARA Mayuka 大分大学, 医学部, 医員 (30774666)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 外尿道括約筋衛星細胞 / ダイレクトリプログラミング / Pax-7 / IL-6 / 長時間トリプシン添付培養 / 再生医療 / 尿失禁

Outline of Final Research Achievements

Our ultimate goal is to develop direct reprogramming of fibroblasts toward external urethral sphincter for future regenerative therapy in urinary incontinence. After analysis of specific transcriptional factors for satellite cells in urethral rhabdosphincter, we plan to deliver these genes into fibloblasts for direct reprogramming toward external urethral sphincter. At the beginning of this project, we have to culture and collect satellite cells in urethral rhabdosphincter effectively.
In this study, we established an effective method to culture and collect satellite cells in urethral rhabdosphincter using long-term trypsin incubation.

Free Research Field

医歯薬学