Development of a novel biosensor for detecting pathogenic bacteria and their related pathogenic factors of periodontal diseases in the gingival sulcus.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26670850
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Health Sciences University of Hokkaido
• Principal Investigator: Endo Kazuhiko 北海道医療大学, 歯学部, 教授 (70168821)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: バイオセンサー / 抗原-抗体反応 / 歯周病原菌

Outline of Final Research Achievements

The objective of this study was to develop a novel biosensor which detects pathogenic bacteria as well as pathogenic factors released from the bacteria for providing a diagnostic method of periodontal diseases.
The titanium sensor on the quartz crystal with K6F peptide antibody was made and measured the concentration of K6F peptide which was one of the degradation products of human gingival fibroblasts by a pathogenic factor (Kgp) released from P. gingivalis. A straight line relation was obtained between the resonant frequency change of the quartz crystal immobilized with K6F peptide antibody and the logarithm of the concentration of K6F peptide in the range from 10 ng/ml to 1,000 ng/ml. The detection limit of K6F peptide for the potential measurement method using the titanium wire sensor immobilized with K6F peptide antibody was approximately 5 mg/ml, which was significantly higher than that for quartz crystal microbalance method.

Free Research Field

生体材料工学