Study of the cancer stem cell induction from the human iPS cell

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670862
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Gifu University
• Principal Investigator: SHIBATA TOSHIYUKI 岐阜大学, 医学(系)研究科(研究院), 教授 (50226172)
• Co-Investigator(Kenkyū-buntansha): KAWAGUCHI Tomoko 岐阜大学, 医学部附属病院, 医員 (30509815) ; IIDA Kazuki 岐阜大学, 医学部附属病院医員, 助教 (30585237) ; TAMAOKI Naritaka 岐阜大学, 大学院医学系研究科, 助教 (40585303)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: iPS細胞 / ヒト歯髄細胞

Outline of Final Research Achievements

One of final purpose of this study is to establish the safety procedure of iPS cells avoiding the carcinogenesis. Our new knowledges from this study are as follows; In the high efficiency of iPS cells and the induction of high quality iPS cells from Dental Pulu Stem Cells (DPSC), enhancement of epithelial marker (E-cadherin), that is epithelial and mesenchymal translation, and enhancement of DLX4 expression (DLX4 is engaged in the TGF-β signal translation) play an important roles in the iPS cell induction efficiency avoiding malignant progression. Furthermore, we carried out the iPS cell induction under TGF-β stimulation. TGF-β stimulation significantly reduced the efficiency of iPS cell induction without the alternation of smad signals. During the analysis of cancer cell effects on the iPS cells, culture conditioned medium possibly affect the iPS cell nature itself including differentiation ability.

Free Research Field

口腔外科