Induction of mandibular bone and tooth germ from embryo body formation form iPS cells in serum-, and feeder-free culture

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670865
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Hiroshima University
• Principal Investigator: Okamoto Tetsuji 広島大学, 医歯薬保健学研究院(歯), 教授 (00169153)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 無血清培養 / 顎骨・歯胚誘導 / iPS細胞 / センダイウイルスベクター / 無フィーダー培養 / 鎖骨頭蓋異形成症 / Noonan症候群 / 基底細胞母斑症候群

Outline of Final Research Achievements

We have successfully established and long-term cultured iPS cells from the pulp cells and peripheral blood mononuclear cells derived from Cleido cranial dysplasia, Noonan, VonRecklinghousendisease, basal nevoid cell carcinoma syndrome in serum-, feeder-,and integration-free culture. These cells exhibited several pluripotent gene expression and differentiation abilities in vitro and in vivo.The teratoma tissues derived from CCD-iPS cells and Noonan-iPA cells exhibited abnormal cartilage structures similar to the patient's cartilage. These results strongly suggest that disease-specific iPS cells might be very useful to elucidate mechanism these diseases.

Free Research Field

口腔外科学