2016 Fiscal Year Final Research Report
Regulation of epithelial polarization to regenerate tooth
| Project/Area Number |
26670880
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Orthodontics/Pediatric dentistry
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | エナメル質 / ３次元培養 / 石灰化 |
|---|
| Outline of Final Research Achievements |
Enamel formation is known as a process that involves cellular proliferation, differentiation through sequential epithelial-mesenchymal interactions, secretion of tissue-specific matrix proteins (e.g., amelogenin), active conveyance of inorganic ions including calcium, and the onset of precipitation and orientation of enamel apatite cystals with enamel matrices. To form artificial enamel, in vitro differentiation system of ameloblast is useful to regenerate tooth and hard tissue. Here, we established new 3D culture system to induce ameloblast differentiation and calcification. A novel 3D culture for dental epithelial cell line SF2 induced enamel specific extracellular matrix, a meloblastin and amelogenin. Further, calcification was observed in this culture system indicating that this new culture method might be useful to form artificial enamel in vitro.
|
| Free Research Field |
小児歯科
|
