Regenerative three dimensional salivary grand from human gingival epithelial stem cell

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670908
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Social dentistry
• Research Institution: The Nippon Dental University
• Principal Investigator: ISHIKAWA Hiroshi 日本歯科大学, 生命歯学部, その他 (30089784)
• Co-Investigator(Kenkyū-buntansha): NAKAHARA Taka 日本歯科大学, 生命歯学部, 教授 (10366768) ; OHYAMA Akihiro 日本歯科大学, 生命歯学部, 准教授 (90538232) ; TOYOMURA Junko 日本歯科大学, 生命歯学部, 助教 (80645630) ; KAWAKAMI Miyuki 日本歯科大学, 新潟生命歯学部, 助教 (40707981) ; TOMINAGA Noriko 日本歯科大学, 生命歯学部, 助教 (90546532)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: ヒト唾液腺 / 歯肉上皮幹細胞 / 共培養 / 3次元的唾液腺 / 唾液腺介在部 / HIF-1alpha / VEGF / 移植

Outline of Final Research Achievements

First, induction decided to make a salivary gland differentiate from a stem cell of the BFP which has no infection. I succeeded in this, then an isolation an epithelial stem cell from a fragment of a gingival tissue by the Friedenstein method. In order to induced differentiation salivary gland epithelial cell, co-cultivation performed a human salivary gland origin fibroblast and isolated epithelial stem cell of gingival tissue. I succeeded to get salivary epithelial cell, then we try to build a three-dimensional salivary gland by a circumfusion culture. We isolated intercalated duct from these reconstructed salivary gland under a microscope. A three-dimensional salivary gland can be built voluntarily from this interstitial segment. It was revealed that the intercalated duct produces a great deal of HIF-1 α and VEGF. Now, fragment of intercalated duct was implanted under the oral mucous membrane of scid mouse.

Free Research Field

再生医療の基礎研究