Principle and application of skin blotting method

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670914
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Fundamental nursing
• Research Institution: The University of Tokyo
• Principal Investigator: MINEMATSU Takeo 東京大学, 医学(系)研究科(研究院), 講師 (00398752)
• Co-Investigator(Kenkyū-buntansha): SANADA Hiromi 東京大学, 大学院医学系研究科, 教授 (50143920) ; MORI Taketoshi 東京大学, 大学院医学系研究科, 教授 (20272586) ; NAKAGAMI Gojiro 東京大学, 大学院医学系研究科, 講師 (70547827) ; NOGUCHI Hiroshi 東京大学, 大学院医学系研究科, 講師 (50431797) ; TAMAI Nao 東京大学, 大学院医学系研究科, 助教 (80636788)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: スキンアセスメント / 褥瘡 / スキンケア

Outline of Final Research Achievements

(1)The principle of skin blotting: We could not fully reveal the principle of skin blotting within this period. Conventional techniques are not enough for the analysis of the effect of skin blotting on the inter-corneocyte lipid lamellae structure. Therefore, we developed new fixation technique of skin tissue, which can keep the structures of inter-corneocyte lipid, epidermis and dermis. Furthermore, the temporal structural change by skin blotting was suggested by immunofluorescence for claudin-1, a tight junction protein.
(2)In the animal experiment, we identified the biomarkers for early detection of pressure ulcer development by skin blotting. The biomarkers are PAI1, VEGF-C, IL1a, and HSP90a, which reflected the ischemia, lymphatic dysfunction, ischemia-reperfusion injury, and deformation of tissue, respectively.

Free Research Field

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