2016 Fiscal Year Final Research Report
Intracellular oxygen imaging using small ratiometric oxygen probes
| Project/Area Number |
26702011
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Gunma University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | りん光 / レシオ / イリジウム錯体 / 酸素 / 蛍光 / 顕微鏡 |
|---|
| Outline of Final Research Achievements |
We designed and synthesized ratiometric molecular probes consisting of a blue fluorescent coumarin and a red phosphorescent cationic iridium complex connected by oligo proline of oligo arginine linkers, respectively, for real time sensing of oxygen levels in living cells. The introduction of oligo arginine linkers improved dramatically the cellular uptake efficiency compared to that of oligo proline linkers. We succeed the detection of oxygen levels in living cells using our small ratiometric oxygen probes.
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| Free Research Field |
光化学
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