Possible mechanism of virus inactivation by disinfections based on proteome analysis

2016 Fiscal Year Final Research Report

• Project/Area Number: 26709037
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Civil and environmental engineering
• Research Institution: Kyoto University
• Principal Investigator: Yasuhiro Asada 京都大学, 工学(系)研究科(研究院), 助教 (60610524)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 上水道 / 健康関連微生物 / 消毒処理 / ウイルス不活化 / プロテオーム解析

Outline of Final Research Achievements

The objectives of this study are to compare and to evaluate inactivation mechanisms by disinfection processes (Chlorination, Ozonation, UV radiation, Advanced Oxidation Process) based on gene analysis and proteome analysis. First, qPCR,PMA-qPCR, ICC-qPCR techniques were employed to determine the inactivation effect. Hydroxyl radicals affected on virus inactivation. And Ozonation is more effective on virus inactivation than other disinfection processes. Also, the obtained results of proteome analysis indicated that damages of proteins are different among Hydroxyl radicals , Chlorination, UV radiation, and ozonation.

Free Research Field

上水道工学