Development of 3D Imaging Cell Sorting Method

2017 Fiscal Year Final Research Report

• Project/Area Number: 26709064
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Shinji Sugiura 国立研究開発法人産業技術総合研究所, 生命工学領域, 上級主任研究員 (10399496)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 細胞分離 / ハイドロゲル / 癌 / 細胞培養 / 包埋培養 / 画像解析

Outline of Final Research Achievements

In a conventional cell separation method such as a flow cytometer, cells are separated using a surface antigen as a marker. On the other hand, the markers for many heterogeneous cell populations such as cancer tissues and stem cell culture systems have been undefined yet, and it is expected to develop a method for separating cells by separately analyzing cell functions for each cell. In this study, we developed the "3D imaging cell sorting method" which separates cells using the photodegradable hydrogel using cell morphology as an indicator for cellular function under three dimensional culture.

Free Research Field

工学