Development of the self-replicable cell-free protein synthesis system

2016 Fiscal Year Final Research Report

• Project/Area Number: 26710014
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: System genome science
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Shimizu Yoshihiro 国立研究開発法人理化学研究所, 生命システム研究センター, ユニットリーダー (90401231)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 無細胞タンパク質合成 / リボソーム / tRNA / マイクロ流路

Outline of Final Research Achievements

To achieve the reconstruction of life by a combination of biomolecules, it is considered essential to reconstitute the means for producing proteins themselves in a self-replicable state. In this research, to achieve self-replication of the translation system by itself, we regarded the PURE system, a reconstituted cell-free protein synthesis system, as a fundamental system for the reconstruction of life and developed each technological basis for ribosome reconstitution, tRNA reconstitution, and protein synthesis in a microchannel.

Free Research Field

生化学