Analysis of intracellular pathogenic pathway of Legionella pneumophila

2017 Fiscal Year Final Research Report

• Project/Area Number: 26713016
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Bacteriology (including mycology)
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: Arasaki Kohei 東京薬科大学, 生命科学部, 講師 (70609990)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: レジオネラ / レジオネラエフェクター / Rabタンパク質 / 小胞輸送 / 小胞体

Outline of Final Research Achievements

In this research, we demonstrated following three things. 1: Legionella manipulates Rab33b-Rab6 cascade to escort LCV to the host ER and we identified the candidate effectors that recruits Rab33b to the Legionella containing vacuole (LCV). 2: After internalization into host cells, Legionella blocks delivery of LCV to the lysosomal compartment. In this process, Legionella degrades Rab5 by ubiquitin dependent manner and we identified E3 ligase like effector against Rab5. 3: It is known that Legionella replicates inside host ER. In this process, we demonstrated that Legionella first associates and fuses with smooth ER to evade host ER. Subsequently, Legionella moves to rough ER and starts to replicates. In this traffic, we identified the host protein that requires traffic of Legionella from the smooth to the rough ER. Furthermore, we also identified the candidate effector that subverts this host factor.

Free Research Field

感染症学