Development of novel therapeutic targets for atopic dermatitis by controlling quorum-sensing in Staphylococcus aureus

2015 Fiscal Year Final Research Report

• Project/Area Number: 26713038
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Dermatology
• Research Institution: Chiba University
• Principal Investigator: MATSUOKA YUMI 千葉大学, 医学(系)研究科(研究院), 助教 (10402067)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: アトピー性皮膚炎 / 黄色ブドウ球菌 / クオラムセンシング

Outline of Final Research Achievements

We found that Myd88-/- mice showed no skin inflammation, whereas WT mice showed severe eczematous lesions with the similar epicutaneous colonization of S. aureus. K14-CreMyd88-/- mice, the skin inflammation was also dramatically reduced, indicating the involvement of MyD88 in KC. We next applied S. aureus on Il1r-/- mice. When applied on Il1r-/- mice with IL-36R Ab, the inflammation was significantly reduced, showing additive IL-36 involvement. Upon the infection, little IL-17 were detected in K14-CreMyd88-/-, Il1r-/- with IL-36RAb mice. In accordance with these, Il17-/- mice showed significantly less skin inflammation upon the infection.We also found that an exotoxin beside secreted from S. aureus was essential in our epicutaneous mouse model. Similar with d-toxin, the expression of this exotoxin is also controlled by agr-quorum sensing in S. aureus indicates that controlling the quorum sensing in S. aureus may be an important therapeutic target of atopic dermatitis.

Free Research Field

皮膚科学