Evaluation of the genotoxicity of thermally treated soils using the electrochemical genotoxicity assay based on a SOS/umu test

2016 Fiscal Year Final Research Report

• Project/Area Number: 26740042
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Environmental risk control and evaluation
• Research Institution: University of Toyama
• Principal Investigator: Sazawa Kazuto 富山大学, 大学院理工学研究部(理学), 助教 (80727016)
• Co-Investigator(Renkei-kenkyūsha): WADA Naoya 富山大学, 研究推進機構極東地域研究センター, 教授 (40272893) ; KURAMITZ Hideki 富山大学, 大学院理工学研究部(理学), 教授 (70397165)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 土壌有機成分 / 森林火災 / 多環芳香族炭化水素

Outline of Final Research Achievements

The effect of forest fire can be seen in the physical, chemical and biological properties of soil. Polycyclic aromatic hydrocarbons (PAHs) are produced by the incomplete combustion of soil organic matter, and they induce DNA damage in bacterial cell. The purpose of this study is to evaluate the genotoxicity of thermally treated soils using a new umu test based on hydrodynamic electrochemical detection.
It was found that the genotoxicity of soil drastically increase when samples were heated over 350 ℃. The intensity of genotoxicity showed a good liner correlation with the concentration of three- and four-rings PAHs and Δb* values of thermally treated samples. The change of Δb* value is strongly related to the dehydration and decarboxylation of soil organic matter. The result obtained from this study concluded that the soil with a high organic matter content such as peat soil indicate high genotoxicity risk.

Free Research Field

環境化学