Development of ion conductance microscopy with high spatio-temporal resolution for visualizing nanostructures of bio samples in liquid environment

2016 Fiscal Year Final Research Report

• Project/Area Number: 26790048
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Thin film/Surface and interfacial physical properties
• Research Institution: Kanazawa University
• Principal Investigator: Watanabe Shinji 金沢大学, バイオAFM先端研究センター, 助教 (70455864)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 物理計測・制御 / 走査プローブ顕微鏡

Outline of Final Research Achievements

We have succeeded in capturing topographic images of samples located in liquid environments from over 10 um scale down to the sub-10 nm scale within a few seconds.
Clearly resolved images of biological samples with sub-10-nm vertical and lateral structures demonstrate the capability of scanning ion conductance microscopy for imaging actions in biological systems with high spatial resolution.

Free Research Field

応用物理一般

Academic Significance and Societal Importance of the Research Achievements

ナノピペットを用いる走査型プローブ顕微鏡、走査型イオン伝導顕微鏡の高速化に取り組み、従来の100倍程度高速でイメージングできる装置を開発した。これを用いて、いくつかのバイオ試料のイメージングを行った。本装置は、生細胞などのダメージに弱いバイオ試料の形状のイメージングやその動態を計測するのに有用であると考えられる。