Selective labeling of target protein by using a chemical probe having a fluorogenic reactive group and an oligonucleotide aptamer

2015 Fiscal Year Final Research Report

• Project/Area Number: 26810091
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: Yamaguchi Takao 東京大学, 分子細胞生物学研究所, 助教 (80596601)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: アフィニティーラベル化 / 核酸アプタマー / タンパク質化学修飾

Outline of Final Research Achievements

Chemical probes, consist of an oligonucleotide aptamer and a fluorogenic reactive group, were designed to achieve a specific labeling of protein of interest (POI). Here, we prepared oligonucleotide aptamer for c-Met, a model POI. In addition, several fluorogenic reactive groups were designed and synthesized. Model experiments revealed that one of the designed fluorogenic reactive groups has a suitable stability and reactivity for the POI-specific labeling.

Free Research Field

生体関連化学