2015 Fiscal Year Final Research Report
Analysis for Signaling Networks by Large-scale Monitoring of Phosphorylated Tyrosines
| Project/Area Number |
26830125
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
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| Research Institution | Kyoto University |
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Principal Investigator |
Sugiyama Naoyuki 京都大学, 薬学研究科(研究院), 准教授 (50545704)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | プロテオーム / シグナル伝達 / リン酸化プロテオミクス |
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| Outline of Final Research Achievements |
In this study, a highly selective method for phosphotyrosine (pY)-containing peptides was developed to comprehensively identify pYs and analyze cellular signaling networks based on the data.
By a combination use of metal oxide chromatography and immunoprecipitation using anti-pY antibody or in vitro kinase reaction with recombinant tyrosine kinases, phosphotyrosines in biological samples were successfully identified in large scale. Using this approach, molecular-targeting agents of a cancer were evaluated and regulated phosphorylated sites were determined.
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| Free Research Field |
プロテオミクス
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