2015 Fiscal Year Final Research Report
Analysis of alternative pathway of tRNA maturation and pre-tRNA capping in tRNA maturation
Project/Area Number |
26840005
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Molecular biology
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Research Institution | The University of Tokyo |
Principal Investigator |
OHIRA TAKAYUKI 東京大学, 工学(系)研究科(研究院), 助教 (90727520)
|
Research Collaborator |
TSUTOMU SUZUKI 東京大学, 大学院工学系研究科, 教授 (20292782)
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Project Period (FY) |
2014-04-01 – 2016-03-31
|
Keywords | tRNA / tRNA maturation / 5' capping / nuclear export / RNA modification |
Outline of Final Research Achievements |
We explored novel transporters required for tRNA maturation via alternative pathway in budding yeast, in which tRNA precursors (pre-tRNAs) with immature ends are exported to the cytoplasm for splicing and then imported back to nucleus to be end-matured, and identified the exportin which was required for normal tRNA maturation. In addition, we also analyzed the biosynthetic mechanism and the physiological function of 5'-capping formed at the 5'-termini of pre-tRNAs with 5'-leader sequences (pre-tRNA capping), and revealed that which was formed by a canonical capping machinery and stabilized the pre-tRNAs by preventing 5'-exonucleolytic degradation, indicating that pre-tRNA capping supported the tRNA maturation.
|
Free Research Field |
分子生物学
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