AMP-activated protein kinase regulates the function of tight junction.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26840068
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: Tomoki Yano 大阪大学, 医学(系)研究科(研究院), 助教 (20546121)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 細胞間接着 / タイトジャンクション / 細胞骨格 / AMP活性化プロテインキナーゼ / アドヘレンスジャンクション

Outline of Final Research Achievements

I found that the AMP-activated protein kinase (AMPK) phosphorylates several proteins of tight junction (TJ). I showed that when cingulin, it is tight junctional protein, was phosphorylated by AMPK, the molecular configurational change occurred. By exploiting this conformational alteration regulated by AMPK, I try to design a module that undergoes efficient fluorescence resonance energy transfer (FRET). FRET probe is possible to be a powerful tool to visualize the intracellular nutritional condition by observing the activity of AMPK.

Free Research Field

細胞生物学