2015 Fiscal Year Final Research Report
Mapping neuropeptide neurons in the brain of Drosophila
| Project/Area Number |
26840110
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Animal physiology/Animal behavior
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 神経ペプチド / コネクトミクス |
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| Outline of Final Research Achievements |
Neuropeptides play a key role in regulation of variety of animal behaviors including feeding and learning. To understand anatomical logic behind, we reconstructed in silico brain-wide neuropeptide map in Drosophila melanogaster. To this end, we developed a software pipeline for high-throughput brain registration processing. Combining this with GAL4/UAS gene expression system and confocal microscopy allowed me to visualize and map brain expression of all neuropeptide-specific GAL4s in the standardized brain coordinates, revealing brain neuropeptide ‘hot spots’ as well as inhibitory regulation of rewarding dopamine system by Allatostatin A.
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| Free Research Field |
神経科学
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