Studies on the mechanism of action of goadsporin and generation of new derivatives

2015 Fiscal Year Final Research Report

• Project/Area Number: 26850043
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: The University of Tokyo
• Principal Investigator: Ozaki Taro 東京大学, 農学生命科学研究科, 特任助教 (40709060)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 抗生物質

Outline of Final Research Achievements

Goadsporin is a secondary metabolite produced by Streptomyces sp. TP-A0584, which shows antibiotic activity against actinomycetes. Goadsporin is also known as an inducer of morphogenesis and secondary metabolism in actinomycetes. Previously, it was suggested that goadsporin targeted Ffh, which is an unprecedented target of antibiotics. However, the details of the mechanism of action remains to be elucidated. In this study, to reveal the mechanism of action of goadsporin, crystallization of Ffh was examined. Biochemical assays, including pull-down assay and surface plasmon resonance, were also performed to analyze the interaction between Ffh and goadsporin. As a result, goadsporin showed interaction with Ffh in both assays. These results suggested that the molecular target of goadsporin was Ffh.

Free Research Field

農芸化学