Establishment of monoclonal antibodies that neutralize all HA subtypes of influenza A viruses

2015 Fiscal Year Final Research Report

• Project/Area Number: 26850180
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Kyoto University (2015); The University of Tokyo (2014)
• Principal Investigator: MURAMOTO Yukiko 京都大学, ウイルス研究所, 特定研究員 (70436567)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: インフルエンザウイルス

Outline of Final Research Achievements

The aim of the study is to establish monoclonal antibodies which recognize all subtypes of influenza A virus HAs. First, we assessed virus-like particles of HIV Gag which possess stalk region of HA protein as an immunogen. HA stalk protein was detected on the surface of Gag virus-like particles generated by the expression of both HIV Gag and HA stalk in mammalian cells. However, we found that the amount of the virus-like particles was not sufficient for immunization. Then, we immunized mice with purified influenza A virus particles. After the fusion process of spleen cells with myeloma cells, antibodies against HA have been screened using several subtypes of HA proteins.

Free Research Field

ウイルス学