2015 Fiscal Year Final Research Report
Genetic and biochemical characterization of cellulases from nobel deep-sea cellulolytic bacteria
| Project/Area Number |
26850226
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental agriculture(including landscape science)
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| Research Institution | Japan Agency for Marine-Earth Science and Technology |
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Principal Investigator |
Uchimura Kohsuke 国立研究開発法人海洋研究開発機構, 海洋生命理工学研究開発センター, 技術副主事 (70416006)
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| Research Collaborator |
Deguchi Shigeru
Kobayashi Tohru
Takaki Yoshihiro
Tsudome Mikiko
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | セルラーゼ |
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| Outline of Final Research Achievements |
We have investigated cellulases from deep-sea cellulolytic bacteria. Cellulases used were purified from a culture broth of a deep-sea bacterium or produced by recombinant Escherichia coli harboring cellulase gene(s) derived from genome analysis. Each cellulase degraded cellulose or acid swollen cellulose well under low temperature, weak alkaline region, and the presence of NaCl, nearly like a deep-sea environment. One of the distinguished characters of these cellulases was their higher molecular weights. They were attributed to a glycoprotein or enzyme possessing several unique cellulose-binding domain. Thus, it revealed that the novel cellulolytic bacteria dwelling in deep-sea produce clearly different cellulaeses from those of terrestrial microorganisms.
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| Free Research Field |
酵素
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