2015 Fiscal Year Final Research Report
Development of a novel method to control translation by pseudorotaxane formation targeting on RNA
| Project/Area Number |
26860007
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Chemical pharmacy
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Keywords | 核酸化学 / 人工核酸 / ロタキサン |
|---|
| Outline of Final Research Achievements |
It is expected that forming rotaxane-like architecture on target mRNA will lead to the regulation of gene expression. In this study, we tried to form pseudorotaxane architecture on the target RNA using a pair of pseudorotaxane-forming oligo DNAs (prfODNs) under physiological conditions. The pseudorotaxane formation reaction proceeded in 85% for 5 min, that is, we have developed the high-performance prfODN with high efficiency and yield.
|
| Free Research Field |
生物有機化学
|
