Identification of the glycosylation and generation mechanisms of high-molecular weight type of lipocalin 2

2017 Fiscal Year Final Research Report

• Project/Area Number: 26860173
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General pharmacology
• Research Institution: Jichi Medical University
• Principal Investigator: FUJIWARA Yoko 自治医科大学, 医学部, ポスト・ドクター (50392494)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 腎障害 / バイオマーカー / 糖鎖 / 細胞極性 / 疾患動物モデル

Outline of Final Research Achievements

Urinary glycoprotein lipocalin 2 (LCN2) has been noted as a sensitive biomarker for acute kidney injury. However, LCN2 could be detected in various disorders associated with the nephropathy since LCN2 is expressed in a variety of tissues. Here we try to identify high-molecular weight LCN2-producing tissues and clarify synthesis and secretion mechanisms of urine specific high-molecular weight LCN2. LCN2 was identified as complex-type glycan in urine, serum, and kidney in inflammatory model mice. On the other hand, hybrid-type glycosylated LCN2 was detected in liver. In addition, high-molecular weight LCN2 in urine was more glycosylated than that in serum and was appeared under intraperitoneal inflammation. These results indicate that LCN2 has a variety of glycosylation and the molecular weight of urinary LCN2 could differ among diseases.

Free Research Field

内分泌学