2015 Fiscal Year Final Research Report
Functional analysis of a novel bromodomain protein in cancer cells
| Project/Area Number |
26860206
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathological medical chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 分子標的治療 / 大腸がん / 遺伝子発現 / エピジェネティクス |
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| Outline of Final Research Achievements |
Bromodomain containing 8 (BRD8) is frequently accumulated in colorectal cancer. Although BRD8 has been considered to alter gene expression through epigenetic modification, genes regulated by BRD8 remain largely unknown. In this study, we analyzed the data of a transcriptome, ChIP-seq, and ChIA-PET, and identified a total of 49 genes that are directly regulated by BRD8. Interestingly, the list includes a gene associated with the regulation of an oncogenic signaling pathway. Consistently, knockdown of BRD8 reduced the expression of downstream gene, leading to change in the activity of the signaling pathway. Since BRD8 is a component in TRRAP/TIP60-histone acetyltransferase complex, our data may help for the development of novel strategies to treat human cancer through targeting the acetyltransferase complex.
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| Free Research Field |
分子腫瘍学
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