Clarification of mechanism in induction of DNA methylation using Epstein-Barr virus infection experiment

2015 Fiscal Year Final Research Report

• Project/Area Number: 26860257
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: Chiba University
• Principal Investigator: Matsusaka Keisuke 千葉大学, 医学(系)研究科(研究院), 助教 (40610150)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: エピジェネティクス / 胃癌 / Epstein-Barr virus

Outline of Final Research Achievements

Genome-wide DNA methylation analysis in early phase of Epstein-Barr virus infection revealed how aberrant DNA methylation could be induced around transcription start sites (TSSs) or in genebody and intergenic regions. DNA methylation occurred from the CpG shore or shelves to CpG islands. In expression analysis by RNA-sequencing, dynamic expression alteration of both host cells and EBV could be observed. These findings should be significant clues to elucidate the mechanisms of induction of aberrant DNA methylation.

Free Research Field

エピジェネティクス