Study on the mechanism of action of novel biofilm inhibitor

2017 Fiscal Year Final Research Report

• Project/Area Number: 26860294
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including mycology)
• Research Institution: Jikei University School of Medicine
• Principal Investigator: OKUDA Kenichi 東京慈恵会医科大学, 医学部, 講師 (70624245)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: バイオフィルム / 黄色ブドウ球菌 / 低分子化合物 / 感染症 / スクリーニング / 細胞壁合成

Outline of Final Research Achievements

Biofilm formation by Staphylococcus aureus is involved in chronic medical device-associated infections. In this study, the mechanism of action of anti-biofilm compound-JK (JK1), a compound that inhibits biofilm formation by S. aureus, was analyzed. It was indicated that JK1 increases oxygen respiration activity of the cells and the activity is important for biofilm inhibition. Furthermore, JK1 increased susceptibility of S. aureus to aminoglycosides. It was also revealed that JK1 specifically binds to LytM, which is involved in cell wall turnover of S. aureus. Analysis using LytM mutants suggested that JK1 binds to the active center of LytM.

Free Research Field

細菌学