Analysis of IL-7 niche in bone marrow

2015 Fiscal Year Final Research Report

• Project/Area Number: 26860322
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Immunology
• Research Institution: Kyoto University
• Principal Investigator: Hara Takahiro 京都大学, ウイルス研究所, 助教 (90512301)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 骨髄 / ストローマ細胞 / IL-7

Outline of Final Research Achievements

Hematopoietic stem cells (HSCs) give rise to all types of blood cells including B lymphocytes. HSCs self-renew in bone marrow niches formed by CAR cells and sinusoidal endothelial cells. It has been predicted that interleukin-7 (IL-7)-dependent B lymphopoiesis is supported by osteoblasts in endosteal region, because in vitro differentiated osteoblasts have the potential to produce IL-7. However, very little is understood about the identity of IL-7-expressing cells in vivo. Here, we show that IL-7-expressing cells form a large subset of CAR cells. Conditional IL-7 deletion in CAR cells dramatically reduced pro B cells and pre B cells, whereas IL-7 deletion in osteoblasts has no effect on B lymphopoiesis. Our studies demonstrate that HSC maintenance and B cell lineage differentiation are distinct cell lineage decisions controlled by HSC niches.

Free Research Field

免疫学