2016 Fiscal Year Final Research Report
Analysis of human haematopietic stem cell movement with PNH-type cell.
| Project/Area Number |
26860363
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 骨髄不全 / ヒト造血幹細胞 / PNH形質血球 |
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| Outline of Final Research Achievements |
The regulation of HSC (Haematopietic stem cell) differentiation was assessed by FCM (Flow cytometry) with a marker, PNH (paroxysmal nocturnal hemoglobinuria)-type cell, which was detected in 60 perentage of bone marrow failure patients. As investigated the percentage of PNH-type cell in various lineages of HSPC (haematopoietc stem progenitor cell), PNH-type cells were detected only 10 perentage in CMP (common myelod progenitors) fraction, however, the same percentage was detected in GMP and MEP as myeloid cells in peripheral blood. Additional genetic analysis to check driver mutation genes effect on the selective growth of PNH-type cell revealed the expression of a specific cytokine was enhanced. From the above, I concluded the differentiation of human HSC was regulated by some complicated factors including HSCs or some cytokines in bone marrow.
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| Free Research Field |
血液内科学
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