2015 Fiscal Year Final Research Report
Development of Rapid, Sensitive and Concurrent Multiple Immunoassay of Disease Markers: Construction by Using Fluorescent Magnetic Beads
| Project/Area Number |
26860364
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
|
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| Research Institution | Shinshu University |
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Principal Investigator |
|
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| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Keywords | 蛍光磁性ビーズ / 疾患マーカー / 免疫測定 / 競合測定 / 同時多項目測定 |
|---|
| Outline of Final Research Achievements |
For improvement of rapidity, sensitivity and specificity of disease diagnosis, we aimed development of a concurrent multiple disease marker immunoassay using fluorescent magnetic beads. First, we developed several fluorescent magnetic beads encapsulating different fluorescent pigments respectively. Next, we constructed a competitive immunoassay using a combination of antibody immobilized fluorescent magnetic beads and an antigen coated plate. Furthermore, using two different colored fluorescent magnetic beads, we concurrently measured two disease markers. As the result of a fluorescent crosstalk correction, the net concentration of each disease maker was correctly measured. Using a magnetic collection of fluorescent magnetic beads, the concurrent multiple immunoassay detected the disease markers within 10 min.
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| Free Research Field |
病態検査学
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